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For immunohistochemical staining, the tissue sections were inactivated by endogenous peroxidase with 3% hydrogen peroxide for 10 min and blocked with goat serum for 30 min

This sequestration increases the pool of free G-actin available for cytoskeletal remodeling, enabling cells to reorganize their internal architecture and migrate toward injury sites

Proposed pathways include modulation of multiple neurotransmitter systems (influencing serotonin, dopamine, norepinephrine, GABA, glutamate levels), possible involvement of NMDA receptors, alpha-1 adrenergic signaling (e.g., potentiating pineal acetyltransferase activity), inhibition of somatostatin release via dopaminergic routes, reduced ACTH/cortisol for stress buffering, and stimulated growth hormone or somatoliberin secretion

Synaptogenesis: Promoting the formation of new synaptic connections between neurons