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After 24 h of incubation, the supernatant was collected to quantify lactate dehydrogenase (LDH) release using the CyQuant LDH assay kit (Invitrogen, Cat #C20300), following the manufacturers protocol

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Glutathione is a tripeptide, a small molecule built from three amino acids (cysteine, glycine, and glutamic acid), and it functions as the primary antioxidant inside nearly every cell