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The frequency of immune cell subsets was assessed by staining 1 x 10 6 nucleated cells with CD45 (KO), CD56 (PC7), CD138 (APC) (both Beckman Coulter), CD14 (PerCP), CD19 (APC-H7), CD3 (V450) and CD16 (PE) (all BD Biosciences)

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[60] Researchers also did not discover any gain in muscle strength, which led them to believe that GH merely let the body store more water in the muscles rather than increase muscle growth