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After three washes with PBS-PVA, cortical granules were labeled with 10 g/mL of Alexa Fluor 488-conjugated wheat germ agglutinin (Invitrogen, Grand Island, NY, United States) in PBS-PVA for 30 min in the dark

This microbial imbalance shifts the gut environment toward inflammation and toxin production, in which it increases harmful bacteria and decreases beneficial species like A

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Furthermore, GSH plays a key role in various physiological processes, including signal transduction, cell proliferation, and apoptosis regulation