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The Go-Nt 831 -GFP sequence was PCR-amplified from Go-Nt 31 -GFP, the product cut with KpnI/NotI and used to replace the GFP sequence in pEGFP-N1 cut with the same enzymes

In normo-weight subjects with MetS, there is a strong association of metabolic parameters with VCAM-1 and Asymmetric Dimethylarginine (ADMA) (a NOS inhibitor) [46], and the aggravation of insulin resistance is often accompanied by abnormal elevations of ICAM-1 and VCAM-1 [47], which decrease with the recovery of erectile function and effective treatment in ED patients [48]

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Yuan Z, Liang Z, Yi J, Chen X, Li R, Wu J, Sun Z (2019) Koumine promotes ROS production to suppress hepatocellular carcinoma cell proliferation via NF-B and ERK/p38 MAPK signaling