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Tubulin expression was detected on the same PVDF membrane using anti--tubulin specific monoclonal antibody (1 g/ml Sigma-Aldrich, Ont., CA), followed by HRP-conjugated goat anti-mouse IgG (1:5000 (v/v), BioRad, Ont., CA)

Additionally, through the use of gridded population estimates, the authors determined the annualized numbers of doses of praziquantel treatment needed to prevent morbidity in sub-Saharan Africa countries for spatial targeting of schistosomiasis control interventions

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