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Description

Cell viability To evaluate cell viability, cells were harvested and propidium iodide (12 g/ml, PI) uptake measured by flow cytometric analysis on a FACS Calibur instrument and Cell quest software (BD Biosciences)

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2 Western blot : Target proteins or interacting proteins were detected using specific antibodies

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To mitigate these risks, comprehensive in vitro and in vivo studies evaluating the immunogenic potential of peptide compounds should be conducted before initiating clinical trials

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