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doi: 10.1371/journal.pone.0095319 282 SmithP

Loscos et al., 2008)

2-chloro-10-[3-(4-methyl-1-piperazinyl)propyl]phenothiazine;ethane-1,2-disulfonic Acid 168

After ultrasonication in buffer A (50 mM Tris7.0, 200 mM NaCl, 20 mM imidazole, 10 mM -mercaptoethanol), the clear lysate was loaded into 5 ml his trap HP column (Cytiva) and eluted with 700 mM Imidazole in buffer A by AKTA Pure

The SOD family (Cu/Zn-SOD1 in the cytoplasm, Mn-SOD2 in mitochondria, and extracellular SOD3) converts superoxide anions (O ) into HO and O via dismutation