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While the effect size can be considered small (adjusted R-squared value of 0.026), suggesting that BMI explains only a small portion of the variance in depressive symptoms in our cohort, a linear association between higher weight status and depressive symptoms is particularly alarming from a public health perspective 61 , especially given the estimated rise of prevalence of obesity in the upcoming years 62

FOXO4-DRI at 25 mM concentration selectively induced p53 nuclear exclusion and apoptosis in the senescent Leydig cells, confirmed by Annexin V-FITC/PI apoptosis assays and immunofluorescent staining of Ser15-phospho-p53 foci

Data availability All data are available in the main text or the Supplementary Materials

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