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For gain-of-function studies, full-length MAT2A and PARN coding sequences were inserted into the BR-V-108 lentiviral expression vector

Many solid tumors are characterized by a profoundly immunosuppressive milieu, marked by the infiltration of regulatory T cells (Tregs), myeloid-derived suppressor cells (MDSCs), and tumor-associated macrophages (TAMs), which collectively dampen anti-tumor immune responses and promote immune escape [263,264,265]

Additionally, tenuigenin affects other proteins involved in memory and synaptic plasticity regulation, reducing the expression of histone deacetylase two and hippocalcin in the rat hippocampus, promoting the phosphorylation of cyclic AMP-responsive element-binding protein and the N-methyl-D-aspartate receptor (NMDAR) 2B subunit, thereby enhancing long-term potentiation (LTP) and synaptic plasticity (Lin et al., 2018)

Moreover, ROS scavengers such as N-acetylcysteine (NAC) inhibit curvature without affecting growth, indicating that asymmetric ROS production drives gravitropic curvature by locally restricting root elongation on the lower flank (Singh et al., 2016)