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Glutamate receptor-mediated analgesic activity of NAC was first observed in rodent models [74,75] and this same metabotropic glutamate receptor-mediated analgesic effect of NAC was later demonstrated in human volunteers exposed to laser stimuli pain [76]

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DNA is also a major target, with ROS causing strand breaks and base modifications such as 8-hydroxydeoxyguanosine (8-OHdG), leading to genomic instability and activation of pro-apoptotic pathways [46]

These newly formed vessels have extremely thin walls and lack smooth muscle components, thus hindering proper oxygen delivery and drug transport during tumor treatment