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[DOI] [PMC free article] [PubMed] [Google Scholar] 90.Gosselink IF

Briefly, the thermocycling protocol followed a pre-incubation at 95C for 10 minutes, followed by 45 cycles of 3-Step amplification: 1) denature at 95C for 20 seconds

For cell proliferation assay, the time when cells adhered to the wall was recorded as point 0, then IL-17A + TNF and different concentration of Deu@PEPS or deucravacitinib were incubated continued (24, 48, and 72 h)

Adult mouse astrocyte isolation Brain tissues were aseptically removed, minced and enzymatically digested in HBSS (Gibco, 14175103) containing 50 mg ml 1 collagenase D (Sigma-Aldrich, 11088858001), 100 g ml 1 TLCK trypsin inhibitor (Sigma-Aldrich, T9378), 100 U l 1 DNase I (Sigma-Aldrich, D5025), and HEPES pH 7.2 (Gibco, 15630080) for 1 h at 23 C with shaking