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Lentivirus was generated by transfecting HEK293T cells plated to roughly 60% confluency in a six-well dish with 250 ng of pMD2.G, 750 ng of psPAX2 and 1,000 ng of lentiviral vector containing the gene of interest with 10 l of PEI in serum-free medium

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RSC Adv 7:3250832517 Cheng C-Y, Barro L, Tsai S-T, Feng T-W, Wu X-Y, Chao C-W, Yu R-S, Chin T-Y, Hsieh MF (2021) Epigallocatechin-3-gallate-loaded liposomes favor anti-inflammation of microglia cells and promote neuroprotection

Some may experience minor side effects such as: Redness or swelling at the injection site Temporary soreness or bruising Less commonly, side effects could include: Mild headaches Nausea Itchy skin or rash Should you notice any prolonged side effects, we recommend speaking with your GP or calling NHS 111 for advice

High concentrations of ROS in the ER lumen may also subsequently activate the ER calcium release channel type 1 inositol 1,4,5-trisphosphate receptor (IP3R1), leading to the leakage of calcium from the ER lumen into the cytoplasm