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Exploring the discrepancy between cell culture and animal studies

In order to get insights about such hypothesis, Blanger and collaborators manipulated glucose utilization in neuronal cultures taking advantage of the observation made by Herrero-Mendez and collaborators that overexpression of Pfkfb3 results in an increased glycolytic rate in cultured rat neurons (Herrero-Mendez et al., 2009)

In this 12-week, randomised, double-blind, placebo-controlled, multicentre trial in 279 individuals with MS, the Cannabis extract (maximum daily delta-9-THC dose = 25 mg/day) produced highly significant reductions in muscle stiffness, muscle spasms, and pain relative to placebo together improved sleep quality that was evident at week-4 and maintained throughout the trial