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2.1.4.2 Decision-guided selection criteria: a hierarchy of constraints To implement this framework, we propose a hierarchy centered on three primary engineering determinants: Structural complexity and PTM thresholds: cargoes exceeding 3050 kDa, containing complex disulfide networks, or requiring eukaryotic post-translational modifications (PTMs) should pivot away from genetic fusion toward modular or chemical approaches to prevent catastrophic perturbation of the capsid assembly energy landscape

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