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Enzyme-linked immunosorbent assay To detect binding of complement components or plasminogen, microtiter plates (Nunc MaxiSorp, Thermo Fisher Scientific) were coated with 100 l of purified His 6 -tagged proteins (5 g/ml) or BSA (5 g/ml) in PBS at 4 C overnight 74

For critically ill patients, intestinal-targeted therapy necessitates a delicate balance between efficacy and safety

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Yuan, S., Mason, A