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Nucleic Acids Research 32(5):1792-1797

Date created: 26 May 2021

Total protein was filtered with a 0.22-m membrane (Millipore, Corp., Billerica, MA, USA), and further purification with the kta Explorer 10 protein purification System (GE Healthcare, Piscataway, NJ, USA) used an ion-exchange chromatography column (HP Q HiTrapTM 5 ml, GE Healthcare, Uppsala, Sweden), loading buffer was buffer A, and elution buffer was buffer B (50 mM Tris-HCl, 1 M NaCl)