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After the incubation, 50 L stop solution was added to each well and gently mixed for 10 min

Standard route for most peptides

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Amir et al., MECP2 is an X-linked gene and most affected patients are females, who present stereotypies, motor capability regression and cognitive impairments that reflect in post-mortem brain tissue by reduced dendritic complexity and reduced spine densities in the hippocampus and across all layers of the cortex (Belichenko et al., MECP2 mutations, and their phenotypic severity depends on the type of mutation or the pattern of somatic X-chromosome inactivation in the patient (Chahrour and Zoghbi, Mecp2 knockouts (Belichenko et al., 2005