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Description

Quantitative immunostaining assay was performed by determining the percentage of positively stained cells in four random high-power fields (200) in four adjacent sections

N.XiaW.et al (2024)

Another centrifugation run of the suspension was performed at 4 C for 30 min at 12,000 g, after which the supernatant was discarded and the pellet was reconstituted in 10 mL PBS via simple mixing and stored at 80 C for future use [19]