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Slides were then incubated in BLOXALL blocking solution (VectorLab, SP-6000) for 15 min, washed in PBS and blocked with Animal-Serum Free serum (Cell Signaling, 15019L) diluted in dH 2 O for 45 min

Finally, we discuss the clinical implications for balancing mucus protection with effective drug delivery and outline key factors that could help to speed up the applications of these new technologies in clinical settings

Relative to single-pathway compounds, this combination provides a more refined framework for studying endocrine feedback loops and the coordinated architecture of metabolic signaling