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It can be concluded that the size change of NPs depends on the rupture of disulfide bonds in response to the intracellular reductive microenvironment and protonation of amine groups of PBAE in slightly acidic TME pH

Recent meta-analyses have emphasized the need for larger-scale, rigorously designed randomized controlled trials to comprehensively determine FMT treatment efficacy and safety, while recommending that future research focus on establishing standardized analytical protocols and validating predictive biomarkers (Zhang et al., 2025)