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Check pH: Test your serum's pH with strips or a meter

This situation was first reported in a boy with a deletion of exon 4 of the IGF-I gene (90) resulting in severe fetal and post-natal growth arrest, poor brain development and extreme insulin resistance with compensatory hyperinsulinemia and acanthosis nigricans

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Subsequently, 180 l fresh culture medium and 20 l of samples (0.00585800 M in sterile water) were added per well and incubated for 30 minutes