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Depurination of the N3Ade adduct is instantaneous, whereas depurination of the N7Gua adduct occurs rather slowly [15, 23, 2729]

These plasmids were constructed by the Gibson assembly method using DNA fragments amplified through PCR with the primer sets listed in Supplementary Table 2

Of the 28.9% (n=818) HCV antibody-positive, 93.8% (n=768) had a confirmatory viral load test, 61.7% (n=474) were HCV RNA-positive

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Betaine supplementation has been used for over 40 years to support individuals with genetic defects of folate dependent remethylation because betaine is known to increase methionine concentration and aid homocysteine reduction