glutathione peroxidase and vitamin e Selenium – Human Nutrition 2e Figure 2 from Vitamin E:

The presence of 7KC and 7-OH elevated the secretion of IL-1 and IL-6 in ARPE-19 cells (Dugas et al., 2010)

To quantify the mScarlet fluorescence in individual chromatography fractions, 100 L samples were each transferred to a 96-well Nunc black microwell plate (Thermo Fisher Scientific) and the fluorescence intensities were measured in a Synergy 2 microtiter plate reader with 530/25 nm and 590/35 nm filters for excitation and emission, respectively

doi: 10.1016/j.reprotox.2005.11.004

Dosage: Follow the recommended dosage provided by your healthcare provider to avoid complications and achieve optimal results

Dan Lepard's recipe suggests leaving to double in height (1.5 hours) but I only leave mine about 10-15 minutes (while the oven preheats)