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These mice were crossed with the colon-specific Cre to generate the CDX2-CreER T2 - Apc fl/fl

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Biomedical research (Tokyo, Japan)

TCF-reporter plasmids pTopFlash (TOP) and pFopFlash (FOP), containing three copies of wild-type or mutant TCF-binding sites upstream of a thymidine kinase minimal promoter driving a luciferase gene, were purchased from Upstate Biotechnology (Lake Placid, NY, USA)