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[2] Leisegang K et al

Western blot analysis Cells, post-treatment with the compounds described in the figure legends, were collected in ice-cold Dulbeccos phosphate-buffered saline (PBS, Gibco, 14190144), washed 2x with PBS and lysed with RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.5% Sodium deoxycholate, 0.1% Sodium dodecyl sulfate, 50 mM Tris pH 8) freshly supplemented with benzonase Nuclease (Merck Millipore, 70746) and Halt Protease Inhibitor Cocktail, EDTA-Free (100X) (Thermo Fisher Scientific, 78425)

Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants

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