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In a preferred embodiment of the invention, drug linker conjugates, where the drug moiety comprises a disulfide (SS) linkage and where a free thiol group is connected to the linker moiety, are reacted with a maleimide-functionalized hydrogel at temperatures between room temperature and 4 C., more preferred at room temperature, in a buffered aqueous solution of pH 2-5, preferably pH 2.5-4.5, more preferably pH 3.0-4.0

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This process subsequently contributes to activating the nuclear factor kappaB (NF-B) pathway, upregulated glutamine transporters such as SLC38A2 and SLC7A5, and ultimately increased glutamine metabolism in cancer cells [128]

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